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anti tcf1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti tcf1
    Anti Tcf1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 238 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tcf1+tcf7/TCF1%2FTCF7+Rabbit+mAb/pmc13023788-370-36-42
    Average 96 stars, based on 238 article reviews
    anti tcf1 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Saline:

    Article Title: Salinomycin inhibits porcine epidemic diarrhea virus infection by targeting Wnt/β-catenin pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) is a re-emerging pathogen that causes severe economic losses in the pig industry.. Commercial PEDV vaccines provide limited protection against PEDV virulent strains.. Therefore, the development of novel vaccines and antiviral drugs is urgently required.

    Incubation:

    Article Title: Salinomycin inhibits porcine epidemic diarrhea virus infection by targeting Wnt/β-catenin pathway.
    Article Snippet: Porcine epidemic diarrhea virus (PEDV) is a re-emerging pathogen that causes severe economic losses in the pig industry.. Commercial PEDV vaccines provide limited protection against PEDV virulent strains.. Therefore, the development of novel vaccines and antiviral drugs is urgently required.

    Staining:

    Article Title: TCF1 and LEF1 promote B-1a cell homeostasis and regulatory function
    Article Snippet: Cells were then permeabilized with PERM III buffer (558050, BD) for 30 min on ice and subsequently stained with phosphorylated BCR signalling components for 40 min at room temperature. .. Human samples were treated with human TruStain FcX CD16, CD32 and CD64 antibodies (BioLegend) and then stained with the following anti-human antibodies: CD3-BV785 (HIT3a; 740961, BD Bioscience), CD19-BUV737 (SJ25C1; 612756, BD Bioscience), CD19-PerCP/Cyanine5.5 (SJ25C1; 363016, BioLegend), CD27-PECy7 (M-T271; 560609, BD Bioscience), CD38-BV605 (HIT2; 569699, BD Bioscience), CD43-BV510 (1G10; 563377, BD Horizon), CD43-FITC (1G10; 555475, BD), CD38-BV510 (HIT2; 303540, BioLegend), CD5-APC (UCHT2; 300612, BioLegend), CD5-PE/Cyanine7 (UCHT2; 300622, BioLegend), IgA-PerCP-Vio700 (IS11-8E10; 130-113-478, MACS), CD24-BV711 (ML5; 563401, BD), IgM-BUV395 (G20-127; 563903, BD), TCF1/TCF7 (C63D9; 2203, Cell Signaling Technology), LEF1 (EPR2029Y; ab137872, Abcam), Alexa Fluor-488 donkey anti-rabbit IgG (minimal x-reactivity Poly4064; 406416, BioLegend) and Alexa Fluor-647 donkey anti-rabbit IgG (minimal x-reactivity Poly4064; 406414, BioLegend). .. Flow cytometers (LSRFortessa X-20, FACSAria II and LSR II; BD) and software (CellQuest and FACSDiva; BD) were used for the acquisition of flow cytometric data, and FlowJo software (Tree Star) was used for analysis.

    Article Title: TCF1 and LEF1 promote B-1a cell homeostasis and regulatory function.
    Article Snippet: Cells were then permeabilized with PERM III buffer (558050, BD) for 30 min on ice and subsequently stained with phosphorylated BCR signalling components for 40 min at room temperature. .. Human samples were treated with human TruStain FcX CD16, CD32 and CD64 antibodies (BioLegend) and then stained with the following anti-human antibodies: CD3-BV785 (HIT3a; 740961, BD Bioscience), CD19-BUV737 (SJ25C1; 612756, BD Bioscience), CD19-PerCP/Cyanine5.5 (SJ25C1; 363016, BioLegend), CD27-PECy7 (M-T271; 560609, BD Bioscience), CD38-BV605 (HIT2; 569699, BD Bioscience), CD43-BV510 (1G10; 563377, BD Horizon), CD43-FITC (1G10; 555475, BD), CD38-BV510 (HIT2; 303540, BioLegend), CD5-APC (UCHT2; 300612, BioLegend), CD5-PE/ Cyanine7 (UCHT2; 300622, BioLegend), IgA-PerCP-Vio700 (IS11-8E10; 130-113-478, MACS), CD24-BV711 (ML5; 563401, BD), IgM-BUV395 (G20127; 563903, BD), TCF1/TCF7 (C63D9; 2203, Cell Signaling Technology), LEF1 (EPR2029Y; ab137872, Abcam), Alexa Fluor-488 donkey anti-rabbit IgG (minimal x-reactivity Poly4064; 406416, BioLegend) and Alexa Fluor-647 donkey anti-rabbit IgG (minimal x-reactivity Poly4064; 406414, BioLegend). .. Flow cytometers (LSRFortessa X-20, FACSAria II and LSR II; BD) and software (CellQuest and FACSDiva; BD) were used for the acquisition of flow cytometric data, and FlowJo software (Tree Star) was used for analysis.

    Article Title: TCF1 and LEF1 promote B-1a cell homeostasis and regulatory function.
    Article Snippet: B220-BUV737 (RA3-6B2, BD Horizon #612838), CD19-BV605(6D5, Biolegend #115540), CD23-BV421(B3B4, Biolegend #101621), CD5APC (53-7.3,ebioscience #17-0051-82), IgM-PE-Cy7 (II/41,ebioscience, #25-5790-82), CD19-BUV395(1D3, BD Horizon, #563557), CD3- 5 nature portfolio | reporting sum m ary April 2023 BV650 (17A2, Biolegend, #100229), CD21-BV605(7G6, BD #747763), IgD-PerCP/Cyanine5.5(11-26c.2a, Biolegend, #405710), CD93BV480(AA4.1, BD, #746239), CD93-PE/Cyanine7(AA4.1, Biolegend, #136506), CD24-Pacific Blue(M1/69, Biolegend #101820), CD43BV605(S7, BD, #747726), CD43-BV711 (S7, BD, #740668), CD3-APC-Cy7(17A2,Biolegend #100222), CD4-APC-Cy7(GK1.5, Biolegend, #100414), CD11b-APC-Cy7(M1/70, Biolegend,#101226), CD11b-AF700(M1/70,Biolegend,#101222), TER119-APC-Cy7(TER-119, BD, #560509), Gr1-APC-Cy7(RB6-8C5, Biolegend, #108424), Sca-1-BV421(Ly-6A/E,Biolegend, # 108128), c-Kit-APC(2B8, Biolegend, #105812), CD127-PE-Cy7(A7R34, Biolegend, #135014), CD16/32-PerCP/Cyanine5.5(93, Biolegend, #101324), CD135-PE(A2F10, Biolegend, #135306), CD45.1-BV711(A20, Biolegend, #110739), CD45.2-BUV737 (104, BD, #612779), CD274-BV711(B7-H1, Biolegend, # 124319), CD86-PE-Cy7(GL-1, Biolegend, #105014), FCRL5-AF488(biotechne, FAB6757G), BLNK phosphorylated at Tyr84 (J117-1278, BD, #558442), Erk1/2 phosphorylated at Thr202/Tyr204 (20A, BD, #561991), phosphorylated PLCγ2 (K86-1161, BD, #560134), Syk phosphorylated at Tyr525/526 (C87C1, Cell Signaling Technology, #6485S) and Btk phosphorylated at Tyr551 (M4G3LN, eBioscience, #12-9015-42), c-Myc-AF647(Y69, abcam, ab190560), TCF1/TCF7-AF488(C63D9, Cell Signaling Technology, #6444S),LEF1-AF488(C12A5, Cell Signaling Technology, #8490S), DOPC/CHOL/Fluorescein-DHPE (54:45:1); Rhodamine-DH PE Liposomes(DOPC/CHOL/Rhodamine-DHPE(54:45:1) were used (FormumMax, Sunnyvale,CA, F60103F2-R). .. IL10-PE (JES5-16E3, Biolegend, #505008), IL10-BV421 (JES5-16E3, BD, #563276), CD223(LAG-3)-PE(C9B7W, Biolegend, #125208), CD138-BV605(281-2, Biolegend, #142515),CD138-PE(281-2, Biolegend, #142504), CD43-BV711 (S7, BD, #740668), CD1d-PerCP/Cy5.5 (1B1, Biolegend, #123513), Ki-67-PerCP-eFluorTM710 (SolA15, eBioscience, #46-5698-82), Ig light chain κ-AF700 (RMK-45, Biolegend, #409508),Ig light chain λ-FITC (R26-46, BD,#553434), IgG3-Biotin (RMG3-1, Biolegend, #406803); For human sample staining: TruStain FcX CD16/32/64 antibodies (Biolegend, #422302), CD3-BV785 (HIT3a, BD Bioscience,#740961), CD19-BUV737(SJ25C1, BD Bioscience, #612756), CD19-PerCP/Cyanine5.5 (SJ25C1, Biolegend, #363016), CD27-PECy7(M-T271, BD Bioscience, #560609), CD38-BV605 (HIT2, BD Bioscience, #569699 ), CD43-BV510(1G10, BD Horizon, #563377), CD43-FITC(1G10, BD, #555475), CD38-BV510 (HIT2, Biolegend, #303540), CD5-APC (UCHT2, Biolegend, #300612), CD5-PE/Cyanine7 (UCHT2, Biolegend, #300622), IgA-PerCP-Vio700 (IS11-8E10, MACS, #130-113-478), CD24-BV711 (ML5, BD, #563401), IgM-BUV395 (G20-127, BD, #563903), TCF1/TCF7(C63D9, Cell Signaling Technology,#2203), LEF1(EPR2029Y, Abcam. .. #ab137872), Alexa Fluor 488 Donkey anti-rabbit IgG (minimal x-reactivity Poly4064, Biolegend, #406416), Alexa Fluor 647 Donkey anti-rabbit IgG (minimal x-reactivity Poly4064, Biolegend, #406414), StreptavidinBV605 (Biolegend, #405229).

    Article Title: PGE 2 inhibits TIL expansion by disrupting IL-2 signalling and mitochondrial function
    Article Snippet: .. The following antibodies were used at dilution 1:50 for staining cells for flow cytometry: CD45 (BV570, HI30 Biolegend, 304034), CD4 (BV605, OKT4, Biolegend, 317438), CD8 (BV650, RPA-T8, Biolegend, 301042), Tim3 (APC fire 750, F38-2E2, Biolegend, 345044), CTLA4 (PE, BNI3, Biolegend, 369604), PD-1 (BV421, EH12.2H7, Biolegend, 329920), CD39 (BV711, TU66, BD Bioscience, 563680), Lag3 (AF488, 11C3C65, Biolegend, 369326), Ki67 (PE–Cy7, Ki67, Biolegend, 350526), Ki67 (AF700, B56, BD Bioscience, 561277), CD57 (BV605, QA17A04, Biolegend, 393304), TOX/TOX2 (PE, E6G50, Cell Signaling, 25202), TOX (PE, REA473, Miltenyi, 130-120-716), CD28 (AF700, CD28.2, Biolegend, 302920), CD27 (APC–Cy7, M-T271, Biolegend, 356424), IL-2Rα (FITC, BC96, Biolegend, 302604), CD122 (PE, TU27, Biolegend, 339006), CD132 (APC, TUGh4, Biolegend, 338608), TCF1/TCF7 (AF647, C63D9, Cell Signaling, 6932), CD56 (PE–Cy7, 5.1H11, Biolegend, 362510), CD137 (PE-Cy5, 4B4-1, Biolegend 309808), CD3 (BV510, UCHT1, Biolegend, 300448), CD3 (BV711, UCHT1, BD Bioscience, 563725), CD4 (PE-CF594, RPA-T4, BD Bioscience, 562281), pS6 (PE, cupk43k, eBioscience, 12-9007-42), IFNγ (APC, B27, Biolegend, 506510), TNF (PE–Cy7, MAb11, BD Bioscience, 557647). .. For flow cytometry phenotyping analysis, T cells were washed with phosphate-buffered saline (PBS) and stained with Zombie UV fixable (Biologend, Cat:423107) or LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Thermo Fisher, L34957) for 15 min on ice in PBS.

    Article Title: PGE 2 inhibits TIL expansion by disrupting IL-2 signalling and mitochondrial function.
    Article Snippet: .. The following antibodies were used at dilution 1:50 for staining cells for flow cytometry: CD45 (BV570, HI30 Biolegend, 304034), CD4 (BV605, OKT4, Biolegend, 317438), CD8 (BV650, RPA-T8, Biolegend, 301042), Tim3 (APC fire 750, F38-2E2, Biolegend, 345044), CTLA4 (PE, BNI3, Biolegend, 369604), PD-1 (BV421, EH12.2H7, Biolegend, 329920), CD39 (BV711, TU66, BD Bioscience, 563680), Lag3 (AF488, 11C3C65, Biolegend, 369326), Ki67 (PE–Cy7, Ki67, Biolegend, 350526), Ki67 (AF700, B56, BD Bioscience, 561277), CD57 (BV605, QA17A04, Biolegend, 393304), TOX/TOX2 (PE, E6G50, Cell Signaling, 25202), TOX (PE, REA473, Miltenyi, 130-120-716), CD28 (AF700, CD28.2, Biolegend, 302920), CD27 (APC–Cy7, M-T271, Biolegend, 356424), IL-2Rα (FITC, BC96, Biolegend, 302604), CD122 (PE, TU27, Biolegend, 339006), CD132 (APC, TUGh4, Biolegend, 338608), TCF1/TCF7 (AF647, C63D9, Cell Signaling, 6932), CD56 (PE–Cy7, 5.1H11, Biolegend, 362510), CD137 (PE-Cy5, 4B4-1, Biolegend 309808), CD3 (BV510, UCHT1, Biolegend, 300448), CD3 (BV711, UCHT1, BD Bioscience, 563725), CD4 (PE-CF594, RPA-T4, BD Bioscience, 562281), pS6 (PE, cupk43k, eBioscience, 12-9007-42), IFNγ (APC, B27, Biolegend, 506510), TNF (PE–Cy7, MAb11, BD Bioscience, 557647). .. For flow cytometry phenotyping analysis, T cells were washed with phosphate-buffered saline (PBS) and stained with Zombie UV fixable (Biologend, Cat:423107) or LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Thermo Fisher, L34957) for 15 min on ice in PBS.

    Magnetic Cell Separation:

    Article Title: TCF1 and LEF1 promote B-1a cell homeostasis and regulatory function
    Article Snippet: Cells were then permeabilized with PERM III buffer (558050, BD) for 30 min on ice and subsequently stained with phosphorylated BCR signalling components for 40 min at room temperature. .. Human samples were treated with human TruStain FcX CD16, CD32 and CD64 antibodies (BioLegend) and then stained with the following anti-human antibodies: CD3-BV785 (HIT3a; 740961, BD Bioscience), CD19-BUV737 (SJ25C1; 612756, BD Bioscience), CD19-PerCP/Cyanine5.5 (SJ25C1; 363016, BioLegend), CD27-PECy7 (M-T271; 560609, BD Bioscience), CD38-BV605 (HIT2; 569699, BD Bioscience), CD43-BV510 (1G10; 563377, BD Horizon), CD43-FITC (1G10; 555475, BD), CD38-BV510 (HIT2; 303540, BioLegend), CD5-APC (UCHT2; 300612, BioLegend), CD5-PE/Cyanine7 (UCHT2; 300622, BioLegend), IgA-PerCP-Vio700 (IS11-8E10; 130-113-478, MACS), CD24-BV711 (ML5; 563401, BD), IgM-BUV395 (G20-127; 563903, BD), TCF1/TCF7 (C63D9; 2203, Cell Signaling Technology), LEF1 (EPR2029Y; ab137872, Abcam), Alexa Fluor-488 donkey anti-rabbit IgG (minimal x-reactivity Poly4064; 406416, BioLegend) and Alexa Fluor-647 donkey anti-rabbit IgG (minimal x-reactivity Poly4064; 406414, BioLegend). .. Flow cytometers (LSRFortessa X-20, FACSAria II and LSR II; BD) and software (CellQuest and FACSDiva; BD) were used for the acquisition of flow cytometric data, and FlowJo software (Tree Star) was used for analysis.

    Article Title: TCF1 and LEF1 promote B-1a cell homeostasis and regulatory function.
    Article Snippet: Cells were then permeabilized with PERM III buffer (558050, BD) for 30 min on ice and subsequently stained with phosphorylated BCR signalling components for 40 min at room temperature. .. Human samples were treated with human TruStain FcX CD16, CD32 and CD64 antibodies (BioLegend) and then stained with the following anti-human antibodies: CD3-BV785 (HIT3a; 740961, BD Bioscience), CD19-BUV737 (SJ25C1; 612756, BD Bioscience), CD19-PerCP/Cyanine5.5 (SJ25C1; 363016, BioLegend), CD27-PECy7 (M-T271; 560609, BD Bioscience), CD38-BV605 (HIT2; 569699, BD Bioscience), CD43-BV510 (1G10; 563377, BD Horizon), CD43-FITC (1G10; 555475, BD), CD38-BV510 (HIT2; 303540, BioLegend), CD5-APC (UCHT2; 300612, BioLegend), CD5-PE/ Cyanine7 (UCHT2; 300622, BioLegend), IgA-PerCP-Vio700 (IS11-8E10; 130-113-478, MACS), CD24-BV711 (ML5; 563401, BD), IgM-BUV395 (G20127; 563903, BD), TCF1/TCF7 (C63D9; 2203, Cell Signaling Technology), LEF1 (EPR2029Y; ab137872, Abcam), Alexa Fluor-488 donkey anti-rabbit IgG (minimal x-reactivity Poly4064; 406416, BioLegend) and Alexa Fluor-647 donkey anti-rabbit IgG (minimal x-reactivity Poly4064; 406414, BioLegend). .. Flow cytometers (LSRFortessa X-20, FACSAria II and LSR II; BD) and software (CellQuest and FACSDiva; BD) were used for the acquisition of flow cytometric data, and FlowJo software (Tree Star) was used for analysis.

    Article Title: TCF1 and LEF1 promote B-1a cell homeostasis and regulatory function.
    Article Snippet: B220-BUV737 (RA3-6B2, BD Horizon #612838), CD19-BV605(6D5, Biolegend #115540), CD23-BV421(B3B4, Biolegend #101621), CD5APC (53-7.3,ebioscience #17-0051-82), IgM-PE-Cy7 (II/41,ebioscience, #25-5790-82), CD19-BUV395(1D3, BD Horizon, #563557), CD3- 5 nature portfolio | reporting sum m ary April 2023 BV650 (17A2, Biolegend, #100229), CD21-BV605(7G6, BD #747763), IgD-PerCP/Cyanine5.5(11-26c.2a, Biolegend, #405710), CD93BV480(AA4.1, BD, #746239), CD93-PE/Cyanine7(AA4.1, Biolegend, #136506), CD24-Pacific Blue(M1/69, Biolegend #101820), CD43BV605(S7, BD, #747726), CD43-BV711 (S7, BD, #740668), CD3-APC-Cy7(17A2,Biolegend #100222), CD4-APC-Cy7(GK1.5, Biolegend, #100414), CD11b-APC-Cy7(M1/70, Biolegend,#101226), CD11b-AF700(M1/70,Biolegend,#101222), TER119-APC-Cy7(TER-119, BD, #560509), Gr1-APC-Cy7(RB6-8C5, Biolegend, #108424), Sca-1-BV421(Ly-6A/E,Biolegend, # 108128), c-Kit-APC(2B8, Biolegend, #105812), CD127-PE-Cy7(A7R34, Biolegend, #135014), CD16/32-PerCP/Cyanine5.5(93, Biolegend, #101324), CD135-PE(A2F10, Biolegend, #135306), CD45.1-BV711(A20, Biolegend, #110739), CD45.2-BUV737 (104, BD, #612779), CD274-BV711(B7-H1, Biolegend, # 124319), CD86-PE-Cy7(GL-1, Biolegend, #105014), FCRL5-AF488(biotechne, FAB6757G), BLNK phosphorylated at Tyr84 (J117-1278, BD, #558442), Erk1/2 phosphorylated at Thr202/Tyr204 (20A, BD, #561991), phosphorylated PLCγ2 (K86-1161, BD, #560134), Syk phosphorylated at Tyr525/526 (C87C1, Cell Signaling Technology, #6485S) and Btk phosphorylated at Tyr551 (M4G3LN, eBioscience, #12-9015-42), c-Myc-AF647(Y69, abcam, ab190560), TCF1/TCF7-AF488(C63D9, Cell Signaling Technology, #6444S),LEF1-AF488(C12A5, Cell Signaling Technology, #8490S), DOPC/CHOL/Fluorescein-DHPE (54:45:1); Rhodamine-DH PE Liposomes(DOPC/CHOL/Rhodamine-DHPE(54:45:1) were used (FormumMax, Sunnyvale,CA, F60103F2-R). .. IL10-PE (JES5-16E3, Biolegend, #505008), IL10-BV421 (JES5-16E3, BD, #563276), CD223(LAG-3)-PE(C9B7W, Biolegend, #125208), CD138-BV605(281-2, Biolegend, #142515),CD138-PE(281-2, Biolegend, #142504), CD43-BV711 (S7, BD, #740668), CD1d-PerCP/Cy5.5 (1B1, Biolegend, #123513), Ki-67-PerCP-eFluorTM710 (SolA15, eBioscience, #46-5698-82), Ig light chain κ-AF700 (RMK-45, Biolegend, #409508),Ig light chain λ-FITC (R26-46, BD,#553434), IgG3-Biotin (RMG3-1, Biolegend, #406803); For human sample staining: TruStain FcX CD16/32/64 antibodies (Biolegend, #422302), CD3-BV785 (HIT3a, BD Bioscience,#740961), CD19-BUV737(SJ25C1, BD Bioscience, #612756), CD19-PerCP/Cyanine5.5 (SJ25C1, Biolegend, #363016), CD27-PECy7(M-T271, BD Bioscience, #560609), CD38-BV605 (HIT2, BD Bioscience, #569699 ), CD43-BV510(1G10, BD Horizon, #563377), CD43-FITC(1G10, BD, #555475), CD38-BV510 (HIT2, Biolegend, #303540), CD5-APC (UCHT2, Biolegend, #300612), CD5-PE/Cyanine7 (UCHT2, Biolegend, #300622), IgA-PerCP-Vio700 (IS11-8E10, MACS, #130-113-478), CD24-BV711 (ML5, BD, #563401), IgM-BUV395 (G20-127, BD, #563903), TCF1/TCF7(C63D9, Cell Signaling Technology,#2203), LEF1(EPR2029Y, Abcam. .. #ab137872), Alexa Fluor 488 Donkey anti-rabbit IgG (minimal x-reactivity Poly4064, Biolegend, #406416), Alexa Fluor 647 Donkey anti-rabbit IgG (minimal x-reactivity Poly4064, Biolegend, #406414), StreptavidinBV605 (Biolegend, #405229).

    Flow Cytometry:

    Article Title: PGE 2 inhibits TIL expansion by disrupting IL-2 signalling and mitochondrial function
    Article Snippet: .. The following antibodies were used at dilution 1:50 for staining cells for flow cytometry: CD45 (BV570, HI30 Biolegend, 304034), CD4 (BV605, OKT4, Biolegend, 317438), CD8 (BV650, RPA-T8, Biolegend, 301042), Tim3 (APC fire 750, F38-2E2, Biolegend, 345044), CTLA4 (PE, BNI3, Biolegend, 369604), PD-1 (BV421, EH12.2H7, Biolegend, 329920), CD39 (BV711, TU66, BD Bioscience, 563680), Lag3 (AF488, 11C3C65, Biolegend, 369326), Ki67 (PE–Cy7, Ki67, Biolegend, 350526), Ki67 (AF700, B56, BD Bioscience, 561277), CD57 (BV605, QA17A04, Biolegend, 393304), TOX/TOX2 (PE, E6G50, Cell Signaling, 25202), TOX (PE, REA473, Miltenyi, 130-120-716), CD28 (AF700, CD28.2, Biolegend, 302920), CD27 (APC–Cy7, M-T271, Biolegend, 356424), IL-2Rα (FITC, BC96, Biolegend, 302604), CD122 (PE, TU27, Biolegend, 339006), CD132 (APC, TUGh4, Biolegend, 338608), TCF1/TCF7 (AF647, C63D9, Cell Signaling, 6932), CD56 (PE–Cy7, 5.1H11, Biolegend, 362510), CD137 (PE-Cy5, 4B4-1, Biolegend 309808), CD3 (BV510, UCHT1, Biolegend, 300448), CD3 (BV711, UCHT1, BD Bioscience, 563725), CD4 (PE-CF594, RPA-T4, BD Bioscience, 562281), pS6 (PE, cupk43k, eBioscience, 12-9007-42), IFNγ (APC, B27, Biolegend, 506510), TNF (PE–Cy7, MAb11, BD Bioscience, 557647). .. For flow cytometry phenotyping analysis, T cells were washed with phosphate-buffered saline (PBS) and stained with Zombie UV fixable (Biologend, Cat:423107) or LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Thermo Fisher, L34957) for 15 min on ice in PBS.

    Article Title: PGE 2 inhibits TIL expansion by disrupting IL-2 signalling and mitochondrial function.
    Article Snippet: .. The following antibodies were used at dilution 1:50 for staining cells for flow cytometry: CD45 (BV570, HI30 Biolegend, 304034), CD4 (BV605, OKT4, Biolegend, 317438), CD8 (BV650, RPA-T8, Biolegend, 301042), Tim3 (APC fire 750, F38-2E2, Biolegend, 345044), CTLA4 (PE, BNI3, Biolegend, 369604), PD-1 (BV421, EH12.2H7, Biolegend, 329920), CD39 (BV711, TU66, BD Bioscience, 563680), Lag3 (AF488, 11C3C65, Biolegend, 369326), Ki67 (PE–Cy7, Ki67, Biolegend, 350526), Ki67 (AF700, B56, BD Bioscience, 561277), CD57 (BV605, QA17A04, Biolegend, 393304), TOX/TOX2 (PE, E6G50, Cell Signaling, 25202), TOX (PE, REA473, Miltenyi, 130-120-716), CD28 (AF700, CD28.2, Biolegend, 302920), CD27 (APC–Cy7, M-T271, Biolegend, 356424), IL-2Rα (FITC, BC96, Biolegend, 302604), CD122 (PE, TU27, Biolegend, 339006), CD132 (APC, TUGh4, Biolegend, 338608), TCF1/TCF7 (AF647, C63D9, Cell Signaling, 6932), CD56 (PE–Cy7, 5.1H11, Biolegend, 362510), CD137 (PE-Cy5, 4B4-1, Biolegend 309808), CD3 (BV510, UCHT1, Biolegend, 300448), CD3 (BV711, UCHT1, BD Bioscience, 563725), CD4 (PE-CF594, RPA-T4, BD Bioscience, 562281), pS6 (PE, cupk43k, eBioscience, 12-9007-42), IFNγ (APC, B27, Biolegend, 506510), TNF (PE–Cy7, MAb11, BD Bioscience, 557647). .. For flow cytometry phenotyping analysis, T cells were washed with phosphate-buffered saline (PBS) and stained with Zombie UV fixable (Biologend, Cat:423107) or LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Thermo Fisher, L34957) for 15 min on ice in PBS.

    Recombinase Polymerase Amplification:

    Article Title: PGE 2 inhibits TIL expansion by disrupting IL-2 signalling and mitochondrial function
    Article Snippet: .. The following antibodies were used at dilution 1:50 for staining cells for flow cytometry: CD45 (BV570, HI30 Biolegend, 304034), CD4 (BV605, OKT4, Biolegend, 317438), CD8 (BV650, RPA-T8, Biolegend, 301042), Tim3 (APC fire 750, F38-2E2, Biolegend, 345044), CTLA4 (PE, BNI3, Biolegend, 369604), PD-1 (BV421, EH12.2H7, Biolegend, 329920), CD39 (BV711, TU66, BD Bioscience, 563680), Lag3 (AF488, 11C3C65, Biolegend, 369326), Ki67 (PE–Cy7, Ki67, Biolegend, 350526), Ki67 (AF700, B56, BD Bioscience, 561277), CD57 (BV605, QA17A04, Biolegend, 393304), TOX/TOX2 (PE, E6G50, Cell Signaling, 25202), TOX (PE, REA473, Miltenyi, 130-120-716), CD28 (AF700, CD28.2, Biolegend, 302920), CD27 (APC–Cy7, M-T271, Biolegend, 356424), IL-2Rα (FITC, BC96, Biolegend, 302604), CD122 (PE, TU27, Biolegend, 339006), CD132 (APC, TUGh4, Biolegend, 338608), TCF1/TCF7 (AF647, C63D9, Cell Signaling, 6932), CD56 (PE–Cy7, 5.1H11, Biolegend, 362510), CD137 (PE-Cy5, 4B4-1, Biolegend 309808), CD3 (BV510, UCHT1, Biolegend, 300448), CD3 (BV711, UCHT1, BD Bioscience, 563725), CD4 (PE-CF594, RPA-T4, BD Bioscience, 562281), pS6 (PE, cupk43k, eBioscience, 12-9007-42), IFNγ (APC, B27, Biolegend, 506510), TNF (PE–Cy7, MAb11, BD Bioscience, 557647). .. For flow cytometry phenotyping analysis, T cells were washed with phosphate-buffered saline (PBS) and stained with Zombie UV fixable (Biologend, Cat:423107) or LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Thermo Fisher, L34957) for 15 min on ice in PBS.

    Article Title: PGE 2 inhibits TIL expansion by disrupting IL-2 signalling and mitochondrial function.
    Article Snippet: .. The following antibodies were used at dilution 1:50 for staining cells for flow cytometry: CD45 (BV570, HI30 Biolegend, 304034), CD4 (BV605, OKT4, Biolegend, 317438), CD8 (BV650, RPA-T8, Biolegend, 301042), Tim3 (APC fire 750, F38-2E2, Biolegend, 345044), CTLA4 (PE, BNI3, Biolegend, 369604), PD-1 (BV421, EH12.2H7, Biolegend, 329920), CD39 (BV711, TU66, BD Bioscience, 563680), Lag3 (AF488, 11C3C65, Biolegend, 369326), Ki67 (PE–Cy7, Ki67, Biolegend, 350526), Ki67 (AF700, B56, BD Bioscience, 561277), CD57 (BV605, QA17A04, Biolegend, 393304), TOX/TOX2 (PE, E6G50, Cell Signaling, 25202), TOX (PE, REA473, Miltenyi, 130-120-716), CD28 (AF700, CD28.2, Biolegend, 302920), CD27 (APC–Cy7, M-T271, Biolegend, 356424), IL-2Rα (FITC, BC96, Biolegend, 302604), CD122 (PE, TU27, Biolegend, 339006), CD132 (APC, TUGh4, Biolegend, 338608), TCF1/TCF7 (AF647, C63D9, Cell Signaling, 6932), CD56 (PE–Cy7, 5.1H11, Biolegend, 362510), CD137 (PE-Cy5, 4B4-1, Biolegend 309808), CD3 (BV510, UCHT1, Biolegend, 300448), CD3 (BV711, UCHT1, BD Bioscience, 563725), CD4 (PE-CF594, RPA-T4, BD Bioscience, 562281), pS6 (PE, cupk43k, eBioscience, 12-9007-42), IFNγ (APC, B27, Biolegend, 506510), TNF (PE–Cy7, MAb11, BD Bioscience, 557647). .. For flow cytometry phenotyping analysis, T cells were washed with phosphate-buffered saline (PBS) and stained with Zombie UV fixable (Biologend, Cat:423107) or LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Thermo Fisher, L34957) for 15 min on ice in PBS.



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